mouse tat elisa kit  (Assaypro)

Journal: Small (Weinheim an Der Bergstrasse, Germany)

Article Title: Multifunctional Co‐Delivery Systems with Downregulation of the Novel Target PIM1 in Macrophages to Ameliorate TF‐Mediated Coagulopathy in Sepsis

doi: 10.1002/smll.202412688

Figure Lengend Snippet: PIM1 inhibitor SMI‐4a protects septic mice against coagulation activation and sepsis‐induced acute lung injury. A) Schematic representation of animal experimental procedures. B) Representative western blot membranes and corresponding densitometric analyses of (C) PIM1 in lung tissue (n = 6/group). D) mRNA levels of PIM1 in murine lung tissues (n = 5/group). E) Platelet count in mice (n = 4/group). F–H) Plasma levels of coagulation‐related factors in mice plasma by ELISA, including (F) TAT, (G) D‐dimer, and (H) fibrinogen (n = 4/group). I) The lung sections were subjected to hematoxylin and eosin (HE) staining, F4/80, fibrin, and PIM1 immunohistochemical analysis (n = 4/group; scale bar:50 µm). J) Representative western blot membranes and corresponding densitometric analyses of (K) TF, (L) PAI‐1, (M) Thrombin in lung tissue (n = 6/group). N) mRNA levels of TF in murine lung tissues (n = 5/group). O–Q) mRNA levels of (O) IL‐1β, (P) IL‐6, and (Q) TNF‐ɑ in murine lung tissues (n = 5/group). R) Survival curves of mice in all groups (n = 10/group). Each bar represents the mean ± SD. Statistical analysis for three or more groups was carried out using one‐way ANOVA (C‐H and K‐Q). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: The concentrations of PIM1 (CSB‐ E11825 h, Cusabio, China) in human plasma and TAT (CSB‐ E08433 m, Cusabio, China), Fbg (CSB‐ E08202 m, Cusabio, China), and D2D (CSB‐ E13584 m, Cusabio, China) in mouse plasma were assessed using ELISA kits according to the guidelines outlined in the respective ELISA kits.

Techniques: Coagulation, Activation Assay, Western Blot, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Staining, Immunohistochemical staining